Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Intersection genes expression and Kaplan-Meier survival analysis based on TCGA clinical cohorts. A was the Venn plot of overlapped DEGs of GSE3678, GSE29265, GSE50901, and GSE33630; B-D were box plot of expression of SLC34A2, SLC4A4, and SLC25A15 in tumor and matched normal thyroid; E-G were the survival curves of SLC34A2, SLC4A4, and SLC25A15, respectively. Black box symbolized the tumor, grey box symbolized the normal. Red line symbolized high expression, blue line symbolized low expression. TCGA: The Cancer Genome Atlas; DEGs: differentially expressed genes.

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques: Expressing

Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Expression of SLC34A2 (A and D), SLC4A4 (B and E), and SLC25A15 (C and F) in tumor tissues and adjacent normal thyroid tissues. A-C were the mRNA expression analysis by RT-PCR (N=8); D-F were the representative protein bands of western blotting. “*” represented P < 0.05.

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Association between the expression of SLC34A2 with clinicopathological characteristics of PTC patients (mean ± SD, n, %)

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques: Expressing

Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Logistic regression analysis for predictors of the capsular invasion risk of PTC patients

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques:

Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Logistic regression analysis for predictors of the extra-thyroid metastasis risk of PTC patients

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques:

Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Logistic regression analysis of high UIC for high SLC34A2 expression risk of PTC patients

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques: Expressing

Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Representative immunohistochemical staining for SLC34A2 and SLC4A4 in tumor tissues and adjacent normal thyroid tissues of PTCs who suffered with capsule invasion and extra-thyroid metastasis, respectively. Scale is 400× for each picture. PTC, papillary thyroid carcinoma.

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques: Immunohistochemical staining, Staining

Journal: Journal of Cancer

Article Title: SLC34A2 Up-regulation And SLC4A4 Down-regulation Correlates With Invasion, Metastasis, And The MAPK Signaling Pathway In Papillary Thyroid Carcinomas

doi: 10.7150/jca.56730

Figure Lengend Snippet: Correlation of SLC34A2 and SLC4A4 expression with MAPK signaling pathway of PTCs. A and B namely the correlation between p-ERK with SLC34A2 (r=0.225, P=0.420) and SLC4A4 (r=-0.354, P=0.215); C and D namely the correlation between p-JNK with SLC34A2 (r=0.437, P=0.118) and SLC4A4 (r=-0.696, P=0.004); E and F namely the correlation between p-P38 with SLC34A2 (r=0.336, P= 0.240) and SLC4A4 (r=-0. 534, P=0.049). PTC, papillary thyroid carcinoma.

Article Snippet: The membranes were blocked with 5% skim milk for 1.5 h, followed by incubation overnight at 4°C with primary antibodies against SLC34A2 (dilution 1:1000; D6W2G, CST, US), SLC4A4 (dilution 1:1000; ab187511, Abcam, UK), SLC25A5 (dilution 1:1000; ab228604, Abcam, UK), ERK (dilution 1:500; bsm-33337M, Bioss, China), p-ERK (dilution 1:500; bs-1522R, Bioss, China), JNK (dilution 1:500; E-AB-60070, Elabscience, China), p-JNK (dilution 1:500; BM4380, Boster, China), P38 (dilution 1:500; E-AB-32459, Elabscience, China), p-P38 (dilution 1:500; E-AB-21027, Elabscience, China), β-actin (dilution 1:2000; 17AV0412, Zhongshan Golden Bridge, China).

Techniques: Expressing

Journal: FEBS open bio

Article Title: SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling.

doi: 10.1002/2211-5463.12594

Figure Lengend Snippet: Fig. 1. SLC34A2 expression is negatively correlated with the overall survival and event-free survival of neuroblastoma patients. (A–F) KM- Plotter analysis showed the correlation between SLC34A2 expression and overall survival (A–C) and relapse-free survival (D–F) of neuroblastoma patients.

Article Snippet: ChIP-grade primary antibody against SLC34A2 (cat. no. 66445) was purchased from Cell Signaling Technology.

Techniques: Expressing

Journal: FEBS open bio

Article Title: SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling.

doi: 10.1002/2211-5463.12594

Figure Lengend Snippet: Fig. 3. SLC34A2 directly binds to the promoter of MIR25 and increases its expression in neuroblastoma cells and spheroids. (A) Diagram of MIR25 promoter containing SLC34A2 binding site and corresponding mutant site. (B) MiR-25 level was examined in SH-SY5Y cells and spheroids. (C) MiR-25 level was determined in SH-SY5Y cells with SLC34A2 overexpression or spheroids with SLC34A2 knockdown. (D) The luciferase activity of pGL3-miR-25 and pGL3-miR-25-mut was measured in SH-SY5Y cells with or without SLC34A2 overexpression. (E) The luciferase activity of pGL3-miR-25 and pGL3-miR-25-mut was measured in SH-SY5Y cells with or without SLC34A2 knockdown. (F) ChIP assay was performed in SH-SY5Y cells with anti-SLC34A or IgG, followed by determination of miR-25 level. The difference was assayed using one-way ANOVA with the Tukey–Kramer post hoc test. Data are presented as mean SD; n ≥3, **P < 0.01 vs control.

Article Snippet: ChIP-grade primary antibody against SLC34A2 (cat. no. 66445) was purchased from Cell Signaling Technology.

Techniques: Expressing, Binding Assay, Mutagenesis, Over Expression, Knockdown, Luciferase, Activity Assay, Control

Journal: FEBS open bio

Article Title: SLC34A2 promotes neuroblastoma cell stemness via enhancement of miR-25/Gsk3β-mediated activation of Wnt/β-catenin signaling.

doi: 10.1002/2211-5463.12594

Figure Lengend Snippet: Fig. 5. SLC34A2 attenuates the stemness of neuroblastoma cells through miR-25–Gsk3b axis. (A) MiR-25 and Gsk3b expression was detected in SH-SY5Y cells with SLC34A2 overexpression plus miR-25 knockdown or Gsk3b overexpression. (B,C) Expressions of stemness markers were measured in cells depicted in (A). (D,E) The capacity of spheroid formation was determined in cells described in (A) by detecting the spheroid size (D) and number (E). Scale bar, 100 lm. (F,G) Expression of Wnt3a and b-catenin was examined in cells depicted in (A). The difference was assayed using one-way ANOVA with the Tukey–Kramer post hoc test. Data are presented as mean SD; n ≥3, *P < 0.05, **P < 0.01 vs control.

Article Snippet: ChIP-grade primary antibody against SLC34A2 (cat. no. 66445) was purchased from Cell Signaling Technology.

Techniques: Expressing, Over Expression, Knockdown, Control

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 + breast cancer stem cells.

doi: 10.1177/1010428317695927

Figure Lengend Snippet: Figure 2. CD147+ spheres express high level of SLC34A2 and SLC34A2 promotes the ability of sphere formation and cell viability. (a) Relative expression of SLC34A2 in CD147+ BCSCs, their differentiated adherent progeny, and adherent cell line cells was examined by qPCR. Columns, mean of three individual experiments; SD, **p < 0.01. (b) Relative expression of SLC34A2 in CD147+ BCSCs (sph), their differentiated adherent progeny (dif), and adherent cell line cells (adh) was examined by western-blot. (c) qPCR was used for selection of si-SLC34A2 stable expressed cell strains. Columns, mean of three individual experiments. (d) Phase- contrast images of CD147+ BCSCs with lentiviral-si-SLC34A2 knockdown (si-SLC34A2), lentiviral-si-NC knockdown (si-NC), and CD147+ BCSCs without treatment (Non-T). Scale bar, 100 µm. (e) The viability of CD147+ BCSCs and CD147+ BCSCs transduced with si-SLC34A2 or empty vector (si-NC) within 7 days was measured by MTT. Columns, mean of three individual experiments; SD, **p < 0.01.

Article Snippet: Membranes were incubated with blocking buffer for 60 min at room temperature and were then incubated with a specific primary antibody against SLC34A2 (catalog No.: 66445; Cell Signal Technology, USA), phospho-AKT (Ser473)(catalog No.: 4070; Cell Signal Technology), PI3K (catalog No.: 4249; Cell Signal Technology), β-catenin (catalog No.: 8480; Cell Signal Technology), Notch-1 (catalog No.: 3608; Cell Signal Technology), AKT (catalog No.: 4685; Cell Signal Technology), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (catalog No.: 5174; Cell Signal Technology) with Blotto overnight at 4°C.

Techniques: Expressing, Western Blot, Selection, Knockdown, Transduction, Plasmid Preparation

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 + breast cancer stem cells.

doi: 10.1177/1010428317695927

Figure Lengend Snippet: Figure 3. High level of SLC34A2 promotes tumorigenicity and the expression of stem cell–associated genes in CD147+ BCSCs. (a) The potential of tumor initiation of CD147+ BCSCs and CD147+ BCSCs transduced with si-SLC34A2 (si-SLC34A2-BCSCs) or empty vector (si-NC-BCSCs) were measured by subcutaneous injection. Representative tumor growth curves of xenografts derived from different cell subpopulations were determined. Columns, mean of three individual experiments; SD, **p < 0.01. (b) qPCR analysis was performed to determine the stem cell–associated gene expression in CD147+ BCSCs transduced with si-SLC34A2 (si-SLC34A2-BCSCs) or empty vector (si-NC-BCSCs). Columns, mean of three individual experiments; SD,**p < 0.01; SD,*p < 0.05.

Article Snippet: Membranes were incubated with blocking buffer for 60 min at room temperature and were then incubated with a specific primary antibody against SLC34A2 (catalog No.: 66445; Cell Signal Technology, USA), phospho-AKT (Ser473)(catalog No.: 4070; Cell Signal Technology), PI3K (catalog No.: 4249; Cell Signal Technology), β-catenin (catalog No.: 8480; Cell Signal Technology), Notch-1 (catalog No.: 3608; Cell Signal Technology), AKT (catalog No.: 4685; Cell Signal Technology), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (catalog No.: 5174; Cell Signal Technology) with Blotto overnight at 4°C.

Techniques: Expressing, Transduction, Plasmid Preparation, Injection, Derivative Assay, Gene Expression

Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Article Title: The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 + breast cancer stem cells.

doi: 10.1177/1010428317695927

Figure Lengend Snippet: Figure 6. SLC34A2 regulates Sox2 through PI3K/AKT cell signaling pathways to maintain the stemness of CD147+ BCSCs. With or without the presence of LY294002, western-blot analysis was performed to determine the expression of SLC34A2, PI3K, AKT, p-AKT, and SOX2 in CD147+ BCSCs and CD147+ BCSCs transduced with si-SLC34A2 or si-SOX2. GAPDH was used as a normalization control.

Article Snippet: Membranes were incubated with blocking buffer for 60 min at room temperature and were then incubated with a specific primary antibody against SLC34A2 (catalog No.: 66445; Cell Signal Technology, USA), phospho-AKT (Ser473)(catalog No.: 4070; Cell Signal Technology), PI3K (catalog No.: 4249; Cell Signal Technology), β-catenin (catalog No.: 8480; Cell Signal Technology), Notch-1 (catalog No.: 3608; Cell Signal Technology), AKT (catalog No.: 4685; Cell Signal Technology), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (catalog No.: 5174; Cell Signal Technology) with Blotto overnight at 4°C.

Techniques: Protein-Protein interactions, Western Blot, Expressing, Transduction, Control